Online Colloquium: “Programming with DNA Outside Living Cells: From Gene Circuits to Self-Assembly” — April 15, 2021 at 4 PM
Dr. Vincent Noireaux
School of Physics and Nanotechnology (PAN)
University of Minnesota
Thursday, April 15, 2021 at 4:00 PM EST
Via Video Conference (contact email@example.com for link information)
All interested persons are cordially invited to join the Zoom call.
Cell-free transcription-translation (TXTL) has become a highly versatile experimental environment to construct biochemical systems in vitro by executing either natural or synthetic gene circuits. In particular, TXTL enables interrogating biochemical systems quantitatively and in isolation far from the complexity of real living cells. I will present several experiments that my lab has done recently using an all-E. coli TXTL system. First, I will present this TXTL system, what it is, what it does. In the second part of my talk, I will show examples of dynamical systems directed by gene circuits executed either in test tubes or in microfluidic chips. In the last part of the talk I will show how we construct synthetic cell systems using TXTL and how synthetic cells are convenient to uncover and quantify fundamental aspects of supramolecular assembly.
Vincent Noireaux is a Professor of Synthetic Biology and Biological Physics at the University of Minnesota in the Department of Physics. His lab has developed a novel cell-free transcription-translation (TXTL) system to construct and characterize biochemical systems in vitro such as gene circuits, protein self-assemblies and bottom-up minimal cells. He earned his PhD at the Curie Institute in Paris, and he was a postdoc at The Rockefeller University in New York.